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人脐血源基质细胞对造血细胞归巢植入能力的影响 被引量：2

Effect of hUCBDSCs on homing efficiency and engraftment capability of hematopoietic cells
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摘要 目的探讨人脐血源基质细胞(human umbilical cord blood-derived stromal cells,hUCBDSCs)对造血细胞早期归巢及植入能力的影响。方法 CCK-8法和Transwell法分别比较hUCBDSCs和人骨髓基质细胞(human bone marrow stromal cells,hBMSCs)对脐血单个核细胞(human umbilical cord blood-mononuclear cells,hUCB-MNCs)体外黏附及迁移能力的影响;RT-PCR法检测hUCBDSCs对促归巢相关因子的表达;60Coγ射线致死剂量辐照BABL/c小鼠后分别接受hUCB-MNCs单移植或hUCB-MNCs联合基质细胞共移植,流式细胞仪检测小鼠骨髓人源CD45+细胞植入率;追踪移植后CM-DiI标记的hUCB-MNCs体内迁移情况,并比较各组归巢率。结果 hUCBDSCs和hBMSCs均能促进hUCB-MNCs的黏附,且迁移作用显著强于无基质细胞共培养对照(P<0.05);hUCBDSCs能表达一系列与造血归巢相关的黏附分子和细胞因子;hUCBDSCs在联合hUCB-MNCs共移植中能不同程度提高造血植入率,且当输注的hUCB-MNCs为低剂量(两种细胞按1∶1各移植2×106/只)时这种促进作用最为显著(P<0.01);CM-DiI标记的hUCB-MNCs在体内主要归巢至骨髓和脾脏,hUCBDSCs联合移植后48 h归巢率(43.49±3.06)%显著高于hBMSCs联合移植组[(31.99±7.26)%]和单移植组[(21.46±6.88)%],(P<0.05)。结论 hUCBDSCs在促进造血细胞迁移、归巢和植入方面作用显著。 Effect of hUCBDSCs on homing efficiency and engraftment capability of hematopoietic cells
作者 刘颖 陈幸华 张曦 高蕾 张诚 冯一梅
机构地区 第三军医大学新桥医院血液科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2011年第5期450-454,共5页 Journal of Third Military Medical University
基金 国家自然科学基金(30971109) 重庆市自然科学基金重点项目(CSTC2009BA5011)~~
关键词 人脐血 基质细胞 造血细胞 归巢 植入 human umbilical cord stromal cells hematopoietic cells homing engraftment
分类号 R392.28 [医药卫生—免疫学] R331.14 [医药卫生—人体生理学]
  • 相关文献

参考文献23

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二级参考文献21

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同被引文献10

  • 1Mendez-Ferrer S,Frenette PS Hematopoietic stem cell trafficking: regulated adhesion and attraction to bone marrow mieroenviron- ment. Ann N Y Acad Sci,2007,1116( 11 ) :392-413.
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  • 3Li A,Zhang Q,J iang J,et al. Contransplantation of bone marrow stromal cells transduced with IL27 gene enhances immune reeonsti- tution after allogeneic bone marrow transp lantation in mice. Gene Ther,2006,13 (15) :1178-1187.
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  • 7Hao L,Gao L, Chen XH, et al. Human umbilical cord blood-de- rived stromal cells prevent graft-versus-host disease in mice follow- ing haplo-identical stem cell transplantation. Cytotherapy. 2011,13 ( 1 ) :83-91.
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  • 9刘颖,陈幸华,张曦,郝蕾,高蕾,张诚.人脐血源基质细胞体外扩增方法的改良[J].解放军医学杂志,2009,34(12):1466-1467. 被引量：2
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第三军医大学学报
2011年 第5期

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