高密度脂蛋白保护烫伤大鼠多脏器功能

The protective effect of HDL on organ function of burn rats

目的了解高密度脂蛋白(HDL)对严重烫伤大鼠脏器功能的保护作用及其机制。方法将Wistar大鼠随机分为对照组(15只)、烫伤组(60只)和HDL组(60只)。后2组均造成30%TBSAⅢ度烫伤,伤后常规补液,其中HDL组伤后立即经尾静脉注入HDL(80 mg/kg)。检测各组血清ICAM-1、TNF-α、oxLDL、CK、ALT、AST、BUN和Cr的含量和PCO2、PO2;观察大鼠伤后各时相点的心、肺、肝及肾组织病理学变化。结果烫伤组大鼠伤后各时相点ICAM-1、TNF-α、oxLDL、CK、ALT、AST、BUN、Cr及PCO2均显著高于对照组(P<0.05),PO2显著降低(P<0.05);烫伤组大鼠心、肺、肝及肾组织病理学变化明显,HDL组上述指标变化显著低于烫伤组。结论 HDL对严重烫伤大鼠脏器功能具有保护作用,可能与通过抑制ICAM-1、TNF-α的表达、减轻体内的炎性反应有关。

Objective To explore the protective effect of high density lipoprotein（HDL） on internal organs function of rats with severe burns.Methods One hundred and thirty-five Wistar rats were randomly divided into control（n=15）,burn（n=60,with 30%TBSA full-thickness burn on the back） and HDL[n=60,with the injection of HDL（80 mg/kg） via the caudal vein immediately after burns]groups.The rats in the latter two groups were resuscitated with intraperitoneal isotonic saline.The serum content of ICAM-1 and TNF-α as well as the blood content of PCO_2 and PO_2 of the rats were determined at 12,24,48 and 72 post-burn hours（PBH） and in control group.The pathological changes of cardiac muscle,pulmonary tissue,hepatic tissue,renal tissue of the rats in all groups were observed at 12,24,48 and 72 PBH.Results PCO_2 and the contents of ICAM-1,TNF-α,oxLDL,CK,ALT,AST,BUN and Cr in burn group were significantly higher,but the PO_2 was lower than those of the control group at each time-point（P0.05）.PCO_2 and the contents of ICAM-1,TNF-α,oxLDL,CK,ALT,AST,BUN and Cr in HDL group were significantly lower,but the PO_2 was higher than those in burn group at each time-point（P0.05）.Compared with the burn group,the pathological changes of the cardiac muscle,pulmonary tissue,hepatic tissue,renal tissue injury was markedly alleviated in the HDL group.Conclusion HDL has the protective effect onorgan function of rats with severe burns via suppressing inflammation by reducing the expression of ICAM-1 and TNF-α.