去精索上神经(SSN)和精索下神经(ISN)对大鼠睾丸发育和精子发生的影响

Effects of Denervation of SSN and ISN on Testicular Development and Spermatogenesis in Rat

本试验旨在研究睾丸神经是否参与调控睾丸的发育和精子的发生过程。大鼠出生后10 d(Postnatal day10,PD10)切断睾丸精索上神经(Superior spermatic nerve,SSN)和精索下神经(Inferior spermatic nerve,ISN),然后分别在PD25和PD50时取样分析。结果显示:睾丸去神经不影响PD25时睾丸质量和睾丸大小(P<0.05),但使PD50时的睾丸质量和睾丸尺寸极显著减小,附睾尾精子数极显著降低(P<0.01)。增殖细胞核抗原(Proliferatingcell nuclear antigen,PCNA)免疫组织化学染色显示睾丸去神经不影响PD25时生殖细胞的增殖,但却影响PD50时生殖细胞的增殖。另外RT-PCR结果显示睾丸去神经明显降低PD25时睾丸3β-羟胆固醇脱氢酶1(3β-hydroxysterol dehydrogenase 1,3β-HSD1)mRNA的表达(P<0.05),并明显抑制PD50时3β-HSD1和雄激素结合蛋白(Androgen binding protein,ABP)mRNA表达(P<0.01)以及黄体生成素受体(Luteinizing hormone receptor,LHR)和卵泡刺激素受体(Follicle stimulating hormone receptor,FSHR)mRNA表达(P<0.05)。结果表明,支配睾丸的神经SSN和ISN主要影响睾丸下降后以及青春期睾丸曲细精管的发育和精子的发生。

The experiment was conducted to reveal whether the superior spermatic nerve（SSN） and the inferior spermatic nerve（ISN） were involved in regulating testicular development and spermatogenesis.The present study was thus designed to transect the SSN and ISN of rats on PD10 and then analyzed changes on PD25 and PD50.The results demonstrated that testicular denervation have no obvious influence to testis mass on PD25（P0.05）,but significantly reduced testis mass,cauda epididymal sperm counts on PD50（P0.01）.Proliferating cell nuclear antigen（PCNA） immunohistochemistry staining proved that the denervation had no influence on the proliferarion of germ cells on PD25,but obviously inhibited the proliferation on PD50.In addition,RT-PCR results showed that testis denervation significantly decreased testis 3β-hydroxy sterol dehydrogenase 1（3β-HSD1） on PD25（P0.05）and significantly decreased testis 3β-HSD1 and androgen binding protein（ABP） mRNA levels（P0.01）,luteinizing hormone receptor（LHR） and follicle stimulating hormone receptor（FSHR） mRNA levels on PD50（P0.05）.These results suggest that the testicular nerve supply plays an important role in supporting seminiferous tubule development and spermatogenesis after testis descend to scrotum.