内毒素血症中肝损伤与H_2S的关系及阳离子A的保护效应分析

The Relationship Between Liver Injury and Hydrogen Sulfide in Endotoxemia and Analysis of Protection of Cation A

本研究旨在探讨在内毒素血症中肝脏病理损伤与气体信号分子硫化氢(H2S)的关系及阳离子A(CA)的保护效应。试验将72只SD大鼠随机分为生理盐水(NS)对照组、ET致病组、CA保护组。3组动物经相应处理后分别在第3、4、8、12小时采集血清与肝脏作为检测样本,检测血清中ALT与AST水平变化,采用分光光度法检测肝脏中H2S生成率,采用原位杂交染色与图像分析检测肝脏组织中CSEmRNA的转录水平。结果显示,与对照组比较,ET组血清ALT与AST水平显著升高,CA保护组血清ALT与AST水平则显著低于ET组;ET能够使肝脏组织中H2S生成率显著升高,而CA保护组H2S生成率显著低于ET组;原位杂交图像分析可见ET上调肝脏组织中CSEmRNA的转录水平,而CA能够抑制这种上调效应。研究表明,在内毒素血症的肝脏病理损伤过程中,CSEmRNA的转录水平与H2S的生成率上调,而CA则下调肝脏组织中H2S的水平从而对肝脏损伤发挥了保护作用。

To explore the realationship between liver injury and hydrogen sulfide（H2S） in endotoxemia and protection of Cation A（CA） in rats.Seventy-two adult healthy SD rats were randomly divided into three groups： control group,ET group,CA group.Hepatic tissue and blood serum of three groups were collected for tests at the 3rd,4th,8th and 12th hour after treatment.The concentration of ALT and AST in serum were detected.The production rate of H2S in liver were measured by spectrophotography.The transcriptional level of CSE mRNA were investigated by in situ hybridization and by image analysis.Compared with control group,ALT and AST level in serum of ET group were significantly higher.In CA group,ALT and AST level is significantly lower than that of ET group.The production rate of H2S in liver of ET group were significantly higher than that of control group,while these items in CA group were significantly lower compared with ET group.The transcriptional level of CSE mRNA and the production rate of H2S in liver were significantly up-regulated and the up-regulation were inhibited markedly by CA.These above results suggested the novel gaseous transmitter H2S mediate liver injure in endotoxemia,whereas CA have a significantly pretective effect on inflammatory injure through down-regulation of the CSE/H2S pathway in liver.