摘要
CBL是近年来发现的一类钙信号转导蛋白,CBL-CIPK组成的信号通路在植物应答生物和非生物刺激中发挥重要作用。其中CBL1和相应的CIPK在低钾,渗透压,干旱,机械损伤,及冻伤等环境胁迫中发挥重要作用。通过对沙冬青CBL1表面带电氨基酸定点突变,表面赖氨酸甲基化后电荷消除证明了沙冬青CBL1(AmCBL1)在钙离子存在下的非特异性聚集是由于分子间的电荷相互作用引起,三体蛋白很可能是沙冬青CBL1蛋白发挥功能的单位。通过甲基化可以得到聚合状态均一的蛋白,为CBL1晶体生长奠定了基础。
CBL is a member of newly identified calcium signal transmitting proteins. CBL-CIPK pathway played an essential role in responding to the biotic and abiotic stimuli in plant. CBL1 and its specified CIPKs are the main executors dealing with various environmental stressed such as low potassium, osmotic, drought, wound, and cold. Site-directed mutation of positive charged amino acid residue and methylation of Lys on the surface demonstrated that the non-specific aggregation of CBL1 from Ammopiptanthus mongolicus （ AmCBLI ） was result from the weak charges interaction between different molecules, and the trimer state may probably be the function unit for the AmCBL1 at the present of Ca^2＋. The homogeneous state of methylated AmCBL1 provided a solid foundation for its crystallization.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2011年第2期23-29,共7页
China Biotechnology
关键词
沙冬青
CBL1
环境胁迫
甲基化
点突变
纯化
Ammopiptanthus mongolicus CBL1 Environmental stresses mutation Purification Methylation Site-directed
