Bacillus amyloliquefaciens ES-2发酵产抗菌脂肽消泡剂的筛选及脂肽的提取和纯化

The Selection of Defoamer for the Antimicrobial Lipopeptide Fermentation by Bacillus amyloliquefaciens ES-2 and Extraction and Purification of Lipopeptide

将大豆油、有机硅消泡剂和聚醚类消泡剂3种消泡剂分别用于Bacillus amyloliquefaciens ES-2抗菌脂肽发酵,研究表明大豆油既可以实现消泡,又有利于抗菌脂肽的发酵,使其产量达到了2497.67mg/L。经过提取得到纯度为55.68%的产品,提取率达到88.72%。此外还比较了不同大孔树脂对抗菌脂肽吸附和解吸附效果,发现大孔树脂X-5最适合抗菌脂肽的纯化。纯化的工艺参数为:上样浓度14.4mg/ml、上样速率1ml/min,洗脱速率2ml/min、洗脱剂用量2.5BV,产品的回收率达90.01%,纯度达74.4%。

Three kinds of defoamers--soybean oil, organic silicone and polyether were used for the antimicrobial lipopeptide fermentation by Bacillus amyloliquefaciens ES-2, and the results showed that soybean oil not only function as a good defoamers, but could also enhance lipopeptide production and got a high yield of 2497.67mg/L. After extraction, the purity of the antimicrobial lipopeptide is 55.68% , and the extraction rate reached 88.72%. The effect of adsorption and desorption of different macroporous resin were also studied, and the result indicated that X-5 macroporous resin was the most suitable for the purification of antibacterial lipopeptide. The optimized technological parameters of the purification were as following： loading concentration 14.4mg/ml, flow rate for adsorption lml/min,desorption flow rate 2 ml/min, and elution volume was 2.5 BV. With above conditions, the recovery ratio and purity of product reached 90.01% and 74.4% separately.