内吗啡肽在小鼠树突状细胞的诱导表达

Inducible expression of endomorphins in murine dendritic cells

目的研究内吗啡肽-1和内吗啡肽-2在小鼠树突状细胞的表达。方法从7～8周龄的C57BL/6J小鼠骨髓提取骨髓前体细胞培养,经CD11c(树突状细胞的特异性标记)免疫磁珠分选,获得纯化的树突状细胞。流式细胞仪分析表明,CD11c阳性细胞纯度达95%以上。免疫荧光染色研究内吗啡肽-1和内吗啡肽-2在树突状细胞的表达;酶免疫测定(enzyme immunoassay,EIA)的方法,检测培养树突状细胞上清液中的内吗啡肽-1和内吗啡肽-2的含量。结果免疫荧光染色表明,活化的树突状细胞内可分泌内吗啡肽-1和内吗啡肽-2;酶免疫测定检测结果表明,不同的Toll样受体(toll like receptor,TLR)配体活化树突状细胞促使分泌不同浓度的内吗啡肽-1和内吗啡肽-2。结论活化的树突状细胞可诱导内吗啡肽的表达。

Aim To study the expression of endomorphin（EM）-1 and EM-2 in dendritic cells（DCs）.Methods Bone marrow dendritic cells were extracted from 7-to 8-week-old C57BL/6J mice,purified with anti-CD11c（a specific marker for dendritic cells） antibody-coated magnetic beads and cultured in the presence of GM-CSF.FACS analysis indicated that the purity of DCs was more than 95%.Immunofluorescence staining was used to detect to expression of EMs in DCs.The enzyme immunoassay（EIA）was used to measure the contents of EMs.Results Immunofluorescence staining revealed that EM-1 and EM-2 were expressed and upregulated in DCs activated by LPS.Enzyme immunoassay showed that LPS and other Toll-like receptor（TLR）ligands promoted the secretion of EM-1 and EM-2 from activated DCs.Conclusions LPS can activate DCs and upregulate the expression of EM-1 and EM-2.TLR ligands can promote the secretion of EMs from DCs.