巴戟天糖链对缺氧复氧损伤内皮细胞的血管形成作用研究

Angiogenesis effects of Morinda officinalis oligosaccharides on human umbilical vein endothelial cells induced by hypoxia-reoxygenation injury

目的探讨巴戟天糖链(MOO)对缺氧复氧损伤内皮细胞的促血管形成作用。方法建立人脐静脉内皮细胞(HUVEC)缺氧复氧损伤(H/R)模型,实验分为6组:正常细胞组、H/R模型组、H/R+阳性对照组、H/R+MOO大、中、小剂量组。用倒置显微镜观察细胞形态;流式细胞仪测量在H/R条件下的细胞周期;采用transwell小室迁移实验和小管形成实验检测MOO对体外内皮细胞血管形成的作用。结果①与正常组相比,H/R模型组增加了G2+S期的细胞(P<0.05),与模型组相比,MOO大、中剂量减弱了这种趋势(P<0.05),使细胞状态更接近于正常细胞;②MOO各剂量组细胞迁移数量高于模型对照组(P<0.05);③MOO各剂量组小管形成的面积较模型对照组亦增多(P<0.05)。结论 MOO可以减轻H/R对血管内皮细胞的损伤,抑制细胞凋亡,有效促进H/R损伤的内皮细胞迁移和形成血管。

Aim To investigate the angiogenesis effects of MOO on HUVECS induced by hypoxia-reoxygenation（H/R）injury.Methods The models of H/R injury were made with the 3～8 generation of cultured HUVECS.Cultured HUVECS were divided into six groups： sham group;hypoxia-reoxygenation（H/R） group;H/R＋SBW;H/R＋low dose of MOO（0.15 g·L-1）;H/R＋medium dose of MOO（0.15 g·L-1）;H/R＋high dose of MOO（0.45 g·L-1）.The state of HUVECS was observed with inverted microscope;cell cycle distribution was examined by flow cytometry（FCM）;transwell chamber migration assays were used to calculate the number of migrated cells;matrigel tubule formation assays were employed to assess the area of tube formation.Results Compared with sham group,H/R increased the ratio of G2＋S phase;but the MOO decreased the Ratio of G2＋S phase;the MOO increased the number of cell migration;tube formation in the area of MOO groups was increased.Conclusion MOO can improve the state of cells induced by H/R,inhibit apoptosis and impact the angiogenesis of HUVEC induced by H/R.