子宫内膜样腺癌中MDM2、Livin和Caspase-3蛋白及mRNA的表达

Expressions of MDM2,Livin and Caspase-3 protein and mRNA in endometrial adenocarcinomas

目的探讨鼠双微体基因(MDM2)、凋亡蛋白抑制因子(Livin)、半胱氨酸天冬氨酸特异性蛋白酶3(Caspase-3)蛋白及mRNA在正常子宫内膜、子宫内膜增殖症和子宫内膜样腺癌(EA)中的表达及临床病理学意义。方法采用组织芯片技术结合免疫组化SP法及原位分子杂交方法,检测30例正常子宫内膜、30例单纯性增生、30例复杂伴不典型性增生、72例EA组织中MDM2、Livin、Caspase-3蛋白及mRNA的表达。结果 MDM2、Livin、Caspase-3蛋白及mRNA在EA中的表达率分别为80.6%(58/72)、80.6%(58/72)、33.3%(24/72)及73.6%(53/72)、75.0%(54/72)、27.8%(20/72),MDM2和L ivin表达率明显高于正常增生内膜(P<0.01),而Caspase-3阳性率明显低于正常增生内膜(P<0.01)。MDM2蛋白及mRNA过表达与组织分化、临床分期、浸润深度和淋巴结转移均无相关性;Livin和Caspase-3蛋白及mRNA阳性表达与组织分化有关(P<0.01或P<0.05),与临床分期、浸润深度及淋巴结转移均无相关性。MDM2、Livin、Caspase-3蛋白与相应的mRNA呈正相关;Livin与Caspase-3表达在蛋白及mRNA水平均呈负相关。结论 MDM2、Livin和Caspase-3蛋白及mRNA异常表达与EA的发生发展相关,有望成为EA早期诊断和预后判断的有价值标记物。

Objective To investigate the relationship of the expression of MDM2,Livin and Caspase-3 protein and mRNA in the development of endometrioid adenocarcinoma（EA）.Methods The expression levels of MDM2,Livin and Caspase-3 proteins and mRNA in EA tissues（n=72）,endometrial hyperplasia tissues（n=60） and normal tissues（n=30） were examined by tissue microarray technique,immunohistochemistry（SP method）and in situ hybridization method.Results The positive expression rates of MDM2,Livin and Caspase-3 protein and mRNA in EA were respectively 80.6%（58/72）,80.6%（58/72）,33.3%（24/72） and 73.6%（53/72）,75.0%（54/72）,27.8%（20/72）.The positive rates of both MDM2 and Livin protein and mRNA in EA were higher than that in normal endometrium and endometrial hyperplasia（P0.01）.However,the positive rate of Caspase-3 in EA was lower than that in normal endometrium and endometrial hyperplasia（P0.01）.The positive expressions of MDM2 protein and mRNA were not related to the histological grade,FIGO stage,depth of invasion and lymph node metastasis.The positive expressions of Livin and Caspase-3 protein and mRNA were related to histological grade（P0.01,P0.05）,but they were not related to FIGO stage,depth of invasion and the lymph node metastasis.The expressions of MDM2,Livin and Caspase-3 protein were positively correlated with their mRNA.The expression of Livin was negatively correlated Caspase-3.Conclusion The expressions of MDM2,Livin and Caspase-3 protein and mRNA correlate with the dedvelopment and progression of EA,which may be valuable biomarkers to detect the early carcinogenesis and prognosis of EA.