强直性脊柱炎患者血清Th1、Th2相关细胞因子表达水平的研究

Expression level of Th1,Th2 relative cytokines in ankylosing spondylitis

目的检测强直性脊柱炎(AS)患者外周血中白介素-4(IL-4)、白介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)及干扰素-γ(IFN-γ)表达水平,探讨其在AS发病机制中作用。方法采用ELISA法测定42例AS患者和40例健康对照者外周血中IL-4、IL-12、TNF-α、IFN-γ水平,对有差异的细胞因子与细胞沉降率(ESR)、C反应蛋白(CRP)、疾病活动指数(BASDAI)、病程进行相关性分析。结果 TNF-α、IFN-γ水平高于健康对照组,但差异无统计学意义;IL-4、IL-12水平高于健康对照组,差异有统计学意义(P<0.05);活动期AS患者组IL-4、IL-12、TNF-α、IFN-γ水平高于非活动期组,但差异无统计学意义;IL-4与BASDAI呈正相关,而与ESR、CRP、病程无相关性;IL-12与ESR、CRP、BASDAI、病程均无相关性。结论 IL-4和IL-12的异常分泌可能与AS的发病有关。

Objective To investigate the serum levels of interleukin-4（IL-4）,interleukin-12（IL-12）,tumor necrosis factor alpha（TNF-α） and interferon gamma-γ（IFN-γ） in 42 patients with ankylosing spondylitis（AS） and 40 healthy control.To research the function of cytokines in the pathogenesis of AS.Methods Enzyme linked immunosorbent assay（ELISA）was used to detected serum levels of IL-4,IL-12,TNF-α and IFN-γ.For the correlation analysis between the cytokines which had significantly difference and laboratory parameters or the BASDAI of AS patients.Results The serum levels of IL-4,IL-12 were higher in AS patients than that in healthy controls,and the serum levels of IL-4,IL-12 were significantly difference.The serum levels of TNF-α and IFN-γ were no statistically significantly difference.The serum levels of IL-4,IL-12,TNF-α and IFN-γ were higher in more active AS patients than that in inactive AS.However,the serum levels of IL-4,IFN-γ,TNF-α and IL-12 were no statistically significantly difference.There was only correlation between serum IL-4 and BASDAI,but there is no correlation with other parameters.Conclusion Increased serum IL-4 and IL-12 levels in AS patients suggests that those two cytokines may trigger the inflammatory process in AS.