白花蛇舌草总黄酮防治小鼠溃疡性结肠炎的作用

Experimental study of the total flavonoids of Oldenlendia diflusa on ulcerative colitis in the rats

目的:观察不同剂量白花蛇舌草总黄酮(FOD)对小鼠产生的溃疡性结肠炎(UC)的防治作用,并初步探讨其作用机制。方法:60只小鼠随机分为6组(n=10):正常对照组、模型组、柳氮磺胺吡啶(SASP)治疗组、FOD高、中、低剂量治疗组。正常对照组一直饮用纯化水,后5组自由饮用4%右旋葡聚糖硫酸钠(DSS)水溶液代替日常饮用水制备UC小鼠模型;造模同时,SASP治疗组灌胃SASP500mg.kg-1.d-1,FOD高、中、低剂量治疗组灌胃FOD分别为60,40,26.7mg.kg-1.d-1,正常对照组和模型组每天灌服等量生理盐水作对照。每天观察各组小鼠的临床症状,喂养7d后处死小鼠取结肠组织,观察结肠黏膜损伤情况,同时检测结肠组织中超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)活性、丙二醛(MDA)和一氧化氮(NO)含量。结果:与模型组比较,FOD高、中剂量治疗组症状不同程度的明显改善、结肠黏膜损伤减轻;结肠组织中SOD活性明显升高(P<0.05),MPO、MDA、NO活性或含量不同程度的明显降低(P<0.05);FOD高剂量治疗组与SASP治疗组作用相似。结论:FOD对小鼠UC作用显著,其机制可能与减少自由基生成、抑制脂质过氧化反应和增强抗氧化能力作用有关。

OBJECTIVE To study the effect of total flavonoids of Oldenlendia diflusa （FOD） on ulcerative colitis （UC） in the rats and ith possible mechanism. METHODS 60 rats were randomly divided into 6 groups （n = 10） normal control group, Sulfasalazine （SASP） treated group （500 mg·kg^-1·d^-1 intragastric administration）, high, medium, low dosage FOD treated group （60,40,26.7 mg·kg^-1·d^-1 intragastric administration） and model control group （normal saline intragastric administration）. The rats were fed with 4G dextran sulphate sodium （DSS） solution for inducing ulcerative colitis, The symptoms of rats were observed everyday, then got colon to observe the lesions of colonic mucosa and the levels of SOD, MPO, MDA, NO in co Ionic tissue were tested after 7 days. RESULTS Compared with the model control group,the symptoms and the lesions of colonic mucosa of animal model treated by FOD in high or medium dose were remarkably improved. In the two groups, SOD activity was significantiy higher （P〈0.05 ）, MPO, MDA and NO content were significantly lower than model control group（PM0. 05）. There was no significant difference between the high dosage FOD treated group and and SASP treated group. CONCLUSION The results indicated that significant effects of FOD on UC may attribute to the reduction in free radical ,depression of lipid peroxidation and the enhancement of antioxidant capability.