吡拉西坦与加兰他敏合用治疗阿尔茨海默病的作用探讨

The pharmacological study of compound piracetam and galantamine on Alzheimer's disease

目的:探讨复方吡拉西坦对痴呆大鼠的作用并比较与吡拉西坦、加兰他敏的疗效。方法:70只Wistar大鼠随机分为假手术组,模型组,复方高、中、低剂量组,单方加兰他敏、单方吡拉西坦7个实验组,每组10只。配制吡拉西坦与加兰他敏的高、中、低3种浓度的复方制剂及其两个单方制剂,术后第2天开始,除伪手术组和模型组灌服等容积的纯化水外,其他实验组分别灌服不同剂量的药物,并于术后第9天行跳台实验,连续记录5d成绩,第14天处死大鼠,测定脑组织中乙酰胆碱和乙酰胆碱酯酶的含量,并制作光镜标本观察海马区组织学变化。结果:与模型组和单方组比较,复方高、中剂量组可显著改善痴呆大鼠组的学习成绩,疗效优于单方给药组(P<0.01);提高脑组织乙酰胆碱的含量(P<0.05);海马区颗粒细胞排列整齐,未见明显异常。结论:复方吡拉西坦对阿尔茨海默病的治疗效果明显优于单方用药,为两药临床合用提供实验基础。

OBJECTIVE To investigate the effect of Compound piracetam on Alzheimer＇s disease rats, and compared with piracetam or galantamine use alone. METHODS Seventy normal Wistar rats （male 35,female 35） were randomly divided into seven groups（n = 10）including sham-operated, model, high dose compound piracetam, medium dose compound piracetam, low dose compound piracetam, piracetam and galantamine groups, except the sham-operated and model groups were administrated with distilled water, other groups were administrated with different dose medicine after the second day of the operation. The abilities of learning and memory of rats were investigated by step-down test on the 9th day after the operation, the changes of the pathology and the activity of the Ach in hippocampus were measured on the 14th day after the operation. RESULTS Compared with model and single component group, the learning and memory abilities were significantly improved （P〈0. 05）, and the brain acetylcho content was increased （P〈0.01） in high and medium dose compound piracetam, the granulose cell of hippocampus in high and medium dose compound groups was lined up in order and no abnormal change was found. CONCLUNION Compound piracetam can improve the therapeutic effect on AD superior to the single component.