山里红叶化学成分分离

Isolation of the Constituent of Hawthorn Leaves

目的:研究山里红叶中(Crataegue pinnatifida Bge.var major)的化学成分。方法:用大孔树脂、硅胶柱色谱、聚酰胺柱色谱、薄层色谱、葡聚糖柱色谱等技术进行分离纯化,根据紫外吸收,液相色谱和核磁共振进行结构鉴定。结果:得到化合物牡荆素-2″-O-鼠李糖苷及牡荆素-4″-O-葡萄糖苷。结论:牡荆素-2″-O-鼠李糖苷及牡荆素-4″-O-葡萄糖苷纯度经高效液相色谱归一化法达99%。

Objective：The purpose is to study the compound in the leaves of Crataegue pinnatifida Bge. var major. Methods： Separating the compound with thin layer and column chromatography, purified by maero polymorous resin, and the compound was identified using the high performance liquid chromatography （ HPLC ） and nuclear magnetic resonance （NMR） method. Reaults：Vi- terin - 2＂ -O - rhamnoside and viterin - 4＂ - O - glucoside was obtained at last. Conclusion ：The purity of viterin - 2＂ - O - rhamnoside and viterin- 4＂-0- glucoside was 〉 99% identified by HPLC method.