芪丹通脉片含药血清促血管生成的实验研究

Containing Serum of Qidantongmai Tablet to Promote Angiogenesis Research

目的:探讨芪丹通脉片含药血清对体内外血管生成的促进作用。方法:采用鸡胚绒毛尿囊膜模型(CAM),观测芪丹通脉片含药血清对CAM新生的促进作用;采用流式细胞术检测芪丹通脉片含药血清对内皮细胞周期的影响;采用免疫组织化学染色法检测内皮细胞VEGF的表达。结果:芪丹通脉片能够明显促进CAM血管新生,但弱于bFGF组;芪丹通脉片促进内皮细胞生长,增加细胞周期中S期细胞的数。芪丹通脉片能够上调VEGF的表达。结论:芪丹通脉片具有促进血管生成作用,其机制与促血管内皮细胞增殖以及上调VEGF的表达有关。

Objective ：To disguss the effect of containing serum of Qidantongmai tablets in vivo or In vitro in promoting anglo-genesis. Methods：useThe model of chicken chorioallantoic membrane （CAM） , observe the containing serum of Qidantongmai tablets in promoting CAM;by flow cytometry Analysis containing serum of Qidantongmai tablets on the endothelial cell cycle;use immunohistochemical staining Detecting the expression of endothelial cell＇ s VEGF. Results ： Qidantongmai tablet can significantly promote the CAM angiogenesis, but was weaker than bFGF group;Qidantongmai tablets to promote endothelial cell growth, increasing the number of S phase cells. Qidantongmai tablet can raise the expression of VEGF. Conclusion： Qidantongmai tablets can promote angiogenesis, The mechanism is Related vascular endothelial cell proliferation and increases the expression of VEGF.