赤藓红荧光激发光谱突变的研究

Study on Saltation of Fluorescence Excitation Spectra of Erythrosine

实验测量了10,20,30,40,50和60μg.mL-1六种浓度赤藓红溶液的荧光激发光谱和吸收光谱。发现在浓度为10和20μg.mL-1时,其荧光激发光谱在530 nm处会出现一个明显的激发峰,而当溶液浓度超过30μg.mL-1后,荧光激发光谱线型会发生突变,530 nm处成为谷值位置,并在530 nm两侧出现两个新的激发峰。对比各种浓度赤藓红溶液的吸收光谱,发现其与荧光激发光谱的变化并不一致,在530nm处均为吸收峰,无突变现象。通过数学计算及一系列对比实验的验证,确定是赤藓红的吸收特性以及光谱测量因素共同导致了其激发光谱的突变。研究结果可为进一步探讨赤藓红的理化特性提供指导,为研究物质荧光激发光谱的突变行为提供参考,并能够促进对荧光激发光谱的正确认识和光谱测量方式的改进。

The fluorescence excitation spectra and absorption spectra of six kinds of erythrosine solutions with concentrations of 10,20,30,40,50 and 60 μg·mL-1 were experimentally measured.It was found that the fluorescence excitation peaks are both located at 530 nm significantly when the concentrations of erythrosine solutions are 10 and 20 μg·mL-1.However,the linetype saltation of fluorescence excitation spectrum occurs as the concentration of erythrosine solution is above 30 μg·mL-1.The valley is located at 530 nm and two new peaks appear at both flanks of the valley.Compared with fluorescence excitation spectra,the absorption spectra of erythrosine solutions are without saltation and the peaks are all located at 530 nm.According to calculations and a series of contrast experiments,it was demonstrated that the absorption characteristic of erythrosine and the spectral measurement mode conspire to cause the saltation of fluorescence excitation spectra.The results can provide guidance for further research on physical and chemical properties of erythrosine,and offer help and reference for study on saltation behavior in fluorescence excitation spectra and improvement in spectral measuring mode.