摘要
本实验建立了饲料中利血平的测定方法。采用高效液相色谱仪,乙酸铵缓冲液+乙腈=60+40作为流动相,C18色谱柱,激发波长:280nm,发射波长:360nm。结果表明:在0.01~10μg/mL浓度范围,线性关系良好,r=0.9999。试样中利血平的检出限为0.05mg/kg。本法操作简便,测定结果准确。
A method for determination of reserpine in feeds by high performance liquid chromatograpy(HPLC) was established.The stationary phase was C18 and the mobile phase was ammonium acetate buffer:acetonitrile=60∶40(V∶V).The excitation wavelength was 280 nm,and the emission wavelength was 360 nm.The calibration curve for reserpine was linear in the range of 0.01 ~ 10 μg/mL(r=0.9999).The limit of quantification was 0.05 mg/kg.This method was simple,fast and accurate.
出处
《中国饲料》
北大核心
2011年第6期38-40,共3页
China Feed
关键词
利血平
饲料
高效液相色谱法
reserpine
feed
high performance liquid chromatography