高效液相色谱-蒸发光散色法测定西洋参中脱氧雪腐镰刀菌烯醇和雪腐镰刀菌烯醇的方法研究

Simultaneous Determination of Deoxynivalenol and Nivalenol in Radix Panacis Quinquefolii by High Performance Liquid Chromatography with Evaporative Light Scattering Detection (HPLC-ELSD)

目的:建立西洋参中脱氧雪腐镰刀菌烯醇(DON)和雪腐镰刀菌烯醇(NIV)的高效液相色谱-蒸发光散色同时检测的方法。方法:样品采用甲醇-水(75:25)混合溶剂进行震荡提取,通过多功能净化柱(MFC)进行一次性净化,以C_(18)柱为分离柱,水-乙腈(80:20)混合溶剂为流动相进行高效液相色谱分离和检测。结果:在西洋参样品中,本方法在0.15～20μg·g^(-1)添加范围内的回收率为86.4%～91.5%;相对标准偏差(RSD)为2.3%～7.4%;DON和NIV的最低检测限分别为15和10ng·g^(-1)(S/N=3)。结论:此方法简便、可靠,可用于西洋参中的DON和NIV含量测定。

The study aimed to establish the method for determining simultaneously deoxynivalenol （DON） and nivalenol （NIV） in Radix Panacis Quinquefolii. The method is to extract the sample with a mixture of acetonitrile-water （75：25）. The extract was purified by multifunctional cleanup column. DON and NIV were then separated and determined by reversed-phase HPLC with C18 column and a mixture of water-acetonitrile（80：20） as mobile phase. The results showed that the average recoveries of DON and NIV from the Radix Panacis Quinquefolii spiked at levels of 0.15-20μg·g^-1 ranged from 86.4%-91.5%, with the relative standard deviation less than 8%. The detection limits for DON and NIV were 15 and 10 ng·g^-1 （S/N=3）, respectively. The conclusion is that the content of DON and NIV in the Radix Panacis Quinquefolii were determined for the first time. The established method can be used in determining the contents of multiple DON and NIV in the Radix Panacis Quinquefolii simultaneously.