均匀设计优化桦褐孔菌多糖提取工艺的研究

Optimization of Extraction Process of Polysaccharide from Inonotus obliquus by Uniform Design

目的:优化桦褐孔菌多糖的提取工艺。方法:采用均匀设计分别对影响提取和醇沉的因素进行优化。结果:最佳提取工艺为固液比1:15,提取时间90min,提取次数2次。最佳醇沉工艺为生药浓度1.0g·mL^(-1),乙醇终浓度65%。结论:优化的桦褐孔菌多糖提取工艺和醇沉工艺经济、稳定、合理可行。

This study aimed to optimize the extraction techniques of polysaccharide from Inortotus obliquus by uni- form design. The content of L obliquus polysaccharides was determined by spectrometry method, which takes anhydrous glucose as standard and 5% phenol and sulfuric acid as color-developing agent, in order to investigate the extraction and alcohol-purification technologies of L obliquus polysaccharides. The extraction and alcohol-purification technologies of L obliquus polysaccharides were optimized by employing the uniform design method. Results showed that the best extraction technology was when the L obliquus and water ratio is 1：15. And the extraction should be given twice and 90 minutes for each time. The best alcohol-purification technology was to add alcohol into the concentration of 1.0 g/mL crude herb to 65% with vigorously rabbling. The conclusion is that the extraction and alcohol-purification technologies of L obliquus polysaccharides optimized by uniform design method are economical, stable and efficient.