TP-M13-SSR技术及其在苹果种质资源遗传多样性研究中的应用

TP-M13-SSR Technique and Its Applications in Analysis of Genetic Diversity for Apple Germplasm Resources

利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR,对苹果种质资源的25份地方品种进行了遗传多样性研究,得到其遗传多样性、多态性信息含量和位点杂合度的变化范围分别为0.5032～0.8448、0.3952～0.8268和0.4400～0.9600。UPGMA法聚类分析将25个苹果品种分为2大类,与地理起源和亲缘关系相关。这种方法具有经济、灵敏、高效等优点,在苹果遗传多样性研究中得到成功应用。本文讨论了TP-M13-SSR技术的优缺点,以及在果树种质资源遗传多样性研究中的应用潜力。

An economical detection method of simple sequence repeat with tailed primer M13（TP-M13-SSR）was used in genetic diversity analysis of 25 regional apple cultivars.The ranges of gene diversity,PIC and locus heterozygosity on 5 SSR loci were 0.5032-0.8448,0.3952-0.8268 and 0.4400-0.9600,respectively.UPGMA cluster analysis showed that the apple species were classified into two groups,correlatively with geographic origin and relationship.The method has advantages of high-throughput,sensitiveness,cost-effectiveness and high accuracy.It had been used in genetic diversity analysis of apple successfully.In addition,the merits and demerits of the TP-M13-SSR technique as well as the potential of application in genetic diversity about fruit tree were assessed.