环带拟盘多毛孢发酵液中一个新的联苯类化合物(英文)

A New Biphenyl from the Fermentation Broth of Plant Endophytic Fungus Pestalotiopsis zonata isolated from Cyrtotachys lakka

目的：研究分离于海南红槟榔中环带拟盘多毛孢发酵液的化学成分。方法：利用硅胶柱层析和凝胶柱层析分离纯化得到4个化合物（1~4）,借助光谱学手段鉴定其结构。测试了化合物1对大肠埃希氏菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克来伯氏菌、MRSA耐甲氧西林金黄色葡萄球菌、鲍曼不动杆菌和VRE耐万古霉素屎肠球菌的抑制活性。结果：分离鉴定了4个化合物包括1个新化合物（2＇-乙酰基-4＇,4-二甲氧基联苯-2-醛基）,该化合物对7种细菌株有较弱抑制活性,IC50分别为0.75,0.75,0.82,0.81,0.84,0.90和0.87μm·mL-1.结论：化合物1为新的联苯类化合物,对7种细菌株表现出比较弱的抑制活性。化合物2~4为首次从该种中分离得到。

AIM： To investigate the chemical constituents of the fermentation broth of the plant endophytic fungus Pestalotiopsis zonata isolated from Cyrtotachys lakka in Hainan, China. METHOD： Compounds 1-4 were separated and purified by silica gel chro-matography and Sephadex LH-20. Their structures were elucidated by extensive spectroscopic analysis. Compound 1 was tested for its antibacterial activity against the bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, methicillin resistant Staphylococcus aureus, Acinetobacter baumannii and vancomycin-resistant Enterococcus faecium. RESULTS： Four compounds including one new compound named 2＇-acetyl-4＇, 4-dimethoxybiphenyl-2-carbaldehyde （1） were isolated from this fungus. Their IC50 values are 0.75, 0.75, 0.82, 0.81, 0.84, 0.90 and 0.87 μm·mL-1, respectively. CONCLUSION： Compound 1 was a new biphenyl and it shows weak activity towards those bacteria. Compounds 2-4 were isolated from this fungus for the first time.