摘要
利用S-8树脂分离葛根提取液中的葛根素,以S-8型大孔树脂对葛根素的吸附行为为研究对象,对葛根素的吸附平衡和吸附动力学进行了研究,发现S-8树脂对葛根素的吸附可以用Langmuir等温吸附方程和Freundlich等温吸附方程来描述,动力学研究表明实验所取初始浓度范围下S-8吸附葛根素的吸附速率由液膜控制,遵循Boyd液膜扩散模型。同时,进行动态吸附解吸实验,在室温(25°C)下,洗脱液为80%的乙醇时,洗脱效果较好,洗脱率为84%,一次纯化后所得产品中葛根素纯度为43.75%。
To assess the adsorption properties of puerarin from Pueraria lobata on S-8 resin in the separation procedure and to optimize the column separation parameters, both static and kinetic adsorption of puerarin on S-8 resin were investigated. The results show that static adsorption behavior of puerarin is correlated with Langmuir adsorption isotherm equation, qe = 147.059 ce/（355.206 ＋ ce）, and Freundlich adsorption isotherm equation, qe = 2.544 ce 0.561. Liquid film diffusion is the control step of adsorption kinetics, and the adsorption kinetics behavior follows the Boyd equation. After purification with S-8 resin, the elution efficiency of puerarin is 84%, and the purity of product reaches 43.75% through detection by high-performance liquid chromatography （HPLC）, when the optimum flow rate and concentration of elution are 2.5 mL·min-1 and 80% ethanol at room temperature.
出处
《中国天然药物》
SCIE
CAS
CSCD
北大核心
2011年第2期120-125,共6页
基金
supported by the Natural Science Foundation of Hubei Province (No. 2008CDA060)
the Innovation Team Funding of Hubei Province (No. T200909)
the Outstanding Young and Middle-aged Professionals Project of Hubei Province (No. Q20101510)
the Youth Science Foundation, Wuhan Institute of Technology (No. Q201009)~~
关键词
S-8树脂
葛根素
葛根
吸附
分离
S-8 macroporous resin
Puerarin
Pueraria lobata
Adsorption
Separation
