大鼠耳蜗螺旋神经节细胞的定量观察

Quantitative analysis of spiral ganglion cells in Rosenthal s canal in rats

目的为定量观察SASCO Sprague Dawley(SD)大鼠耳蜗蜗轴螺旋管(Rosenthal's canal)切片中螺旋神经节细胞的数量提供实验依据。方法 11只SD大鼠用于本实验研究,其中5只正常SD大鼠用于对照,另外6只SD大鼠用于制备乌苯苷引起的螺旋神经节细胞损害模型。耳蜗样品制作耳蜗中轴半薄切片,常规甲苯胺蓝染色,对耳蜗各回蜗轴螺旋管内的螺旋神经节细胞进行计数,计数结果采用方差分析检验。结果一个完整蜗轴螺旋管内的螺旋神经节细胞数量在底回末段多于耳蜗底回起始段和中回中段。与正常大鼠耳蜗螺旋神经节数量相比,1mM乌苯苷仅造成耳蜗底回的部分螺旋神经节细胞破坏,而10mM乌苯苷可破坏绝大部分螺旋神经节,乌苯苷引起的螺旋神经节破坏模式是从耳蜗底回逐渐向顶回发展。结论计数耳蜗各回不同部位切片中的蜗轴螺旋管内螺旋神经节细胞数量是一种简便可靠的耳蜗螺旋神经节细胞定量分析方法。

Objective To quantify the number of spiral ganglion neurons （SGN） in sections of Rosenthal＇s canal in different locations in rats. Methods llSASCO Sprague Dawley rats were used for this project. 5 cochleas from normal rats were studied for the normal control data, another 6 rats were treated with lmM ouabain or 10raM ouabain locally via round window membrane as the models of spiral ganglion neuron degeneration. The cochlea was prepared for cochlear semi-thin sections with toluidine blue staining. The number of SGN in sections of Rosenthal＇s canal at different locations was counted. The differences of SGN at various locations were statistically analyzed by analysis of variance （ANOVA）. Results The SGN in a full Rosenthal＇s canal in the upper region of basal turn was more than the bottom of basal turn and the second turn in normal rats. Compare the SGN number with normal control rats, lmM ouabain only damages the SGN in the basal turn of the cochlea, however, 10mM ouabain destroys most SGN in the cochlea. The destruction of SGN was in a stereotypic pattern that damage begins at the base of the cochlea and progresses toward the apex. Conclusions Counting the number of SGN in a full Rosenthal＇s canal section at different locations was a credible and simple method.