摘要
为了研究光动力过程中光剂量和光敏剂剂量对光动力损伤效果的影响,基于肿瘤组织中光动力损伤剂量的数学模型以及光动力损伤剂量与组织中光剂量、光敏剂剂量、氧浓度之间的函数关系,用数学方法模拟研究了给定模型组织中光及光敏剂的有效吸收剂量与氧浓度之间的关系,并用ALA-PDT实验研究了k562细胞悬浮液中不同药物剂量对光动力损伤效果的影响。研究发现,光动力过程中光剂量与光敏剂浓度存在一个最佳治疗剂量,其大小和组织中氧浓度有关,组织中越缺氧,最佳光动力剂量越小,光动力过程对组织的损伤越小,通过实验发现k562细胞悬浮液中最佳剂量为20×0.25(J/cm^2.mmol/L),此时光动力损伤效果最明显。研究结果表明了PDT对肿瘤组织具有选择性光动力损伤的特点,并为PDT在临床上的广泛应用提供了理论支持。
Based on the mathematical model of photodynamic-damage dose in tumor and the function of photodynamic-damage dose with light dose, photosensitizer dose and oxygen concentration, the relationship of effective absorbed dose of light and photosensitizer changed with oxygen concentration is study, then give the photodynamic-damage effects of k562 cells changed with different medicine （light and photosensitizer） dose.The results shows that there is a optimal therapeutic dose through PDT, and its size related with oxygen concentration, the more hypoxia, the smaller the optimal dose ofphotodynamic, and the less the tumor is damaged by using experimental method it was fund that the optimal dose in k562 cells suspension is 20 × 0.25（J/cm2.mmol/L）,at the optimal dose, photodynamic damage effects is the most obvious. It is shown that tumor can be damaged selectively in PDT, and it provides a theoretical support for PDT to be widely used in clinic.
出处
《应用激光》
CSCD
北大核心
2011年第1期92-96,共5页
Applied Laser
基金
国家自然科学基金资助项目(项目编号:60978070)
河南省教育厅自然科学研究计划资助项目(项目编号:2008A180040)
