胰岛素样生长因子Ⅰ基因转移对NOD鼠胰岛炎及胰岛β细胞凋亡的保护作用

Adenovirai vector-mediated IGF- I gene transfer protects NOD mice from diabetes

探讨腺病毒介导的胰岛素样生长因子Ⅰ（Ad-IGF—Ⅰ）对NOD鼠胰岛炎及胰岛β细胞凋亡的影响。结果发现Ad-IGF—Ⅰ可减少糖尿病的累积发病率，降低胰岛炎积分、促进胰岛β细胞增殖，减少β胞凋亡率，使Fas、半胱氨酸天冬氨酸蛋白酶3（caspase-3）的蛋白低表达，Bcl-xl蛋白高表达。提示Ad—IGF—Ⅰ可减少NOD鼠胰岛炎，通过Fas和Bcl-xl途径发挥抗凋亡作用。

[ Summary] To explore the protective effect of adenovirus mediated IGF- Ⅰ gene （ Ad-IGF- Ⅰ ） transfer on non-obese diabetic （ NOD ） mice. The results showed that the incidence of diabetes and degree of insulitis were significantly reduced in mice receiving Ad-IGF- Ⅰ. Treatment with Ad-IGF- Ⅰ significantly decreased apoptosis rate, expression of Fas and caspase-3, and increased expression of Bcl-xl. This study indicates the potential of IGF- Ⅰ gene therapy in protecting NOD mice from insulitis and apoptosis.