IL-23p19 mRNA在RA患者滑膜组织及滑膜细胞中的表达

The Expression of IL-23p19 mRNA in Synovial Tissue and RA Synovial Fibroblast of RA Patients

目的:探讨类风湿性关节炎(RA)与骨性关节炎(OA)患者滑膜组织及体外培养滑膜成纤维细胞(SF)中白细胞介素(IL)-23p19mRNA分泌来源及对RA诊断的意义。方法:收集行滑膜切除术及关节置换术的6例RA患者及6例OA患者滑膜组织,酶消化法对滑膜组织行SF体外培养,RT-PCR方法检测RA和OA滑膜组织、SF中IL-23p19mRNA表达水平,RA患者滑膜组织、SF中IL-1βmRNA的表达水平。结果:(1)6例RA患者和4例OA患者滑膜组织、SF或OASF中IL-23p19mRNA表达均为阳性,且RA患者滑膜组织中的表达高于OA患者,RASF中的表达量显著高于OASF中阳性标本的表达量(均P<0.05)。(2)6例RA患者滑膜组织中IL-1βmRNA表达均阳性,且与IL-23p19mRNA表达水平呈正相关(r=0.900,P<0.05),6例RASF中IL-1βmRNA表达均阳性,且与IL-23p19mRNA表达水平正相关(r=0.883,P<0.05)。结论:IL-23参与了RA的发病过程,并且可由RA患者中的SF分泌,IL-23可作为RA与OA鉴别诊断的指标之一,IL-1β很可能是IL-23在RA中发挥致病作用的途径之一。

Objective： To investigate the secretion source and the diagnostic significance of interleukin（IL）-23 in patients with rheumatoid arthritis（RA）and osteoarthritis （OA）.Methods： The samples of synovial tissues were obtained in 6 RA and 6 OA patients during synovectomy and joint replacement surgery.The samples were extracted by enzyme.RT-PCR was used to detect the expressions of IL-23p19 mRNA,IL-1β mRNA and GAPDH mRNA.Results： （1）The levels of IL-23p19mRNA were positive in synovial tissues and blasts（SF） of 6 RA and 4 OA patients.In all the positive samples,the expression of IL-23p19 mRNA was significant higher in RA patients than that of OA patients （P 〈 0.05）.（2）The expressions of IL-1β mRNA were positive in all synovial tissues of 6 RA patients,which was correlated to the expression of IL-23p19 mRNA（r = 0.900,P 〈 0.05）.The expressions of IL-1β mRNA were positive in all of 6 RASF,and was also correlated to the expression of IL-23p19m RNA in RASF （r = 0.883,P 〈 0.05）.Conclusion： The expression of IL-23 is involved in the pathogenesis of RA.And SF is also involved in this secrete of IL-23 in RA patients.IL-23 may be one of indicators of the differential diagnosis of RA and OA.IL-1β may be one of pathways that IL-23 involved in RA pathogenesis.