mTOR信号通路在胰腺癌发病中的作用

Role of mTOR signaling pathway in the pathogenesis of pancreatic cancer

目的检测mTOR信号通路在胰腺癌组织中的表达，探讨其在胰腺癌发病中的作用。方法选择经手术、病理证实的6例胰腺癌及相应癌旁组织，抽提RNA，应用Agilent人全基因表达谱芯片进行检测，生物信息学分析mTOR信号通路在胰腺癌组织中的表达。结果总共筛选到1276个差异基因，其中癌组织上调的有691个，下调的有585个。KEGG通路的Enrichment和基因计数两项指标得分最高的是mTOR信号通路中的hsa04150，其Enrichment为4．5622519，基因计数为9，基因计数百分率为1．15％，EASE Score P值为6．23E-04，最有生物学意义，其中ULK2、PIK3R3、PDPK1、EIF4EBP1、PGF、VEGFB、ULK3、RICTOR与PIK3R5等9个关键基因具有显著性差异（P值均〈0．05）。结论胰腺癌发病与mTOR信号通路激活密切相关。

Objectives To investigate the expression of roTOR signaling pathway in pancreatic cancer and export its signification. Methods 6 samples of pancreatic cancer and its paracaneerous tissues specimens confirmed by surgery and pathologic examination were selected. RNA was extracted and expression profiles experiment was performed by using Agilent human whole genomic oligonucleotide microarrays. The expression of roTOR signaling pathway in pancreatic cancer was analyzed by bioinformatics. Results Totally 1276 differential gene were selected, and 691 were up-regulated in cancer tissue, while 585 were down-regulated. The highest score of KEGG pathway＇s Enrichment and gene count was hsa04150 in roTOR signaling pathway, with its Enrichment of 4. 5622519 and gene count of 9, and the percentage of gene count was 1. 15%, the EASE Score P value was 6.23 E-04, which had the most biological significance. Among those, there was significantly difference of expression of nine key genes including ULK2, PIK3R3, PDPK1, EIF4EBP1, PGF, VEGFB, ULK3, RICTOR and PIK3R5 （P 〈 0.05）. Conclusions The pathogenesis of pancreatic cancer is related to the activation of the mTOR signaling pathway.