雷奈酸锶对糖皮质激素诱导骨质疏松大鼠骨形态计量学的研究

The effect of strontium ranelate on bone histomorphometry in glucocorticoid-induced osteoporosis in rats

目的观察雷奈酸锶对糖皮质激素诱导骨质疏松大鼠骨形态计量学的影响。方法 24只3.5月龄SPF级雄性SD大鼠适应性喂养1w后,随机等分为3组:Nrm组:正常对照组;Met组:皮下注射甲强龙(Met)5 mg/(kg.d),每周5次;SrR组:在Met组基础上给予雷奈酸锶900 mg/(kg.d)灌胃。实验期9w。第5腰椎(L5)和右侧股骨用于骨密度测定,右侧胫骨行骨形态计量学分析。结果 Met组L5和股骨BMD显著低于Nrm组;SrR组L5和股骨BMD显著高于Met组。Met组BV/TV,Tb.Th,Tb.N,%Ct.Ar显著低于Nrm组,Tb.Sp,%Ma.Ar,ES/BS显著高于Nrm组;SrR组BV/TV,Tb.Th,Tb.N,%Ct.Ar显著高于Met组;Tb.Sp,ES/BS显著低于Met组。结论给予大鼠SrR 900 mg/(kg.d)灌胃,在提高激素诱导骨质疏松大鼠的骨密度,促进骨形成,降低骨吸收,延缓骨量丢失方面有积极作用;对继发性骨质疏松的预防和治疗有一定前景。

Objective To observe the effect of strontium ranelate （SrR） on bone histomorphometry in glucocorticoid-induced osteoporosis in rats. Methods Twenty-four 3.5-month male Sprague-Dawley rats were randomly divided into three groups： age-matched normal control group （Nrm） , methylprednisolone group （Met） in which rats were given subcutaneously Met 5.0 mg/kg per day for 5 days/week, and Met plus SrR group in which rats were given intragastric SrR 900 mg/kg-d in addition to Met. The study lasted for 9 weeks. The right femoral bone and the L5 were taken to examine the bone densitometry and the right tibia was taken for bone histomorphometry analysis. Results BMDs of the L5 and the femoral bone in Met were significantly lower than those in Nrm. BMDs of the L5 and the femoral bone in SrR were significantly higher than those in Met. BV/TV, Tb. Th, Tb. N, and% Ct. Ar in Met were significantly lower than those in Nrm. Tb. Sp,% Ma. Ar, and ES/BS in Met were significantly higher than those in Nrm. BV/TV, Tb. Th, Tb. N, and% Ct. Ar in SrR were higher than those in Met. Tb. Sp and ES/BS in SrR were lower than those in Met. Conclusion SrR 900 mg/kg intragastric administration had a positive effect on increasing BMD, stimulating bone formation, reducing bone resorption, and delaying bone loss in glucocorticoid-induced osteoporosis in rats. SrR might be applied in treating and preventing of secondary osteoporosis.