雷奈酸锶在模拟微重力环境对成骨细胞增殖功能的影响

Effects of strontium ranelate on osteoblast proliferation in simulated microgravity

目的观察新型的抗骨质疏松药雷奈酸锶在模拟微重力环境下对成骨细胞增殖功能的影响。方法利用沿水平轴连续回转(30 r/min)细胞培养系统模拟微重力环境,使用MTT比色法或台盼兰染色、细胞计数法观察小鼠成骨样细胞MC3T3-E1的增殖情况。结果模拟微重力环境可以降低MC3T3-E1增殖功能,雷奈酸锶在模拟微重力环境可以增强MC3T3-E1增殖功能。结论在模拟微重力环境下雷奈酸锶对成骨细胞增殖功能具有保护作用,为雷奈酸锶治疗微重力环境骨量丢失提供了理论和实验证据。

Objective To investigate the role of strontium ranelate, the novel antiosteoporotic agent, in regulating the proliferation of mouse osteoblastic cell MC3T3-E1 in simulated microgravity. Methods Cell proliferation were evaluated using MTT assay or trypan blue staining and viable ceil counting in a rotating cell culture system （30rpm/minutes） that simulated microgravity. Results Simulated microgravity induced a decrease in proliferation of MC3T3-E1 cells and this could be reversed by treatment with strontium ranelate. Conclusion Strontium ranelate played protective role for osteoblast proliferation in simulated microgravity environment. This provides a theoretical and experimental evidence for the treatment of microgravity induced bone loss with strontium ranelate.