摘要
目的探讨雌激素(17β-estradiol,17β-E2)对B段紫外线(UVB)辐射引起皮肤损伤的保护作用及其保护机制。方法体外培养人永生化角质形成细胞(HaCaT),并将其随机分为4组:对照组、UVB组、E2组和E2+UVB组,分别给予不同的处理,经UVB照射后6 h,收集各组细胞上清液,应用酶联免疫吸附试验(ELISA)方法检测UVB照射后6 h各组细胞上清液中白细胞介素10(IL-10)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)分泌量的变化;UVB照射后12 h,收集各组细胞,采用单细胞凝胶电泳技术检测各组细胞的DNA损伤程度。结果与对照组相比较,UVB组IL-10、IL-6和TNF-α的含量显著升高(P<0.05);经E2预处理后,E2+UVB组细胞上清液中IL-10、IL-6和TNF-α的含量均显著降低(P<0.05)。单细胞凝胶电泳检测结果显示,UVB组和E2+UVB组均出现彗星样拖尾,应用CASP和SPSS软件分析统计后得出,UVB组细胞尾部DNA百分含量、尾长和尾矩与对照组相比具有统计学意义;经E2预处理后,UVB对细胞损伤程度明显降低,具有统计学意义(P<0.05)。结论雌激素可降低UVB引起的IL-10、IL-6和TNF-α的产生,并能对抗UVB照射对细胞DNA的损伤,从而减轻紫外线辐射引起的皮肤损伤。
Objective To study the protective mechanism of 17β-estradiol for the HaCaT cells irradiated with ultraviolet radiation B.Methods The HaCaT cells were cultivated in RPMI 1640 and randomly classified into four differently-treated groups： normal cell group,UVB group,E2 group,and E2＋UVB group.The secretion levels of IL-10,IL-6 and TNF-α of cultured HaCaT cells were detected by enzyme-linked immunosorbent assay（ELISA） and DNA damage was measured by single cell gel electrophoresis（SCGE）.Results The content of IL-6,IL-10 and TNF-α in UVB group cells was significantly higher than that in control group（P0.05）.After being preconditioned with E2,the content of IL-6,IL-10 and TNF-α in E2＋UVB group was significantly decreased.SCGE revealed that in UVB group and E2＋UVB group the DNA fragmentation（comet） of cells was induced by UVB.After being assayed by CASP and SPSS,the tail DNA%,tail length and tail moment in UVB group cells were significantly higher than those in control group cells（P0.05）,but after being preconditioned with E2,the DNA damage in E2＋UVB group cells was significatly reduced.Conclusion 17β-Estradiol treatment can relieve the skin damage induced by ultraviolet B by decreasing the secretions of IL-10,IL-6 and TNF-α and reducing the DNA damage.
出处
《军事医学》
CAS
CSCD
北大核心
2011年第2期107-110,共4页
Military Medical Sciences
基金
中华医学会欧莱雅中国人健康皮肤项目(S2008050603)
