热休克蛋白70在急性百草枯中毒大鼠肺脏中的表达

Expression of heat shock protein 70 in lung tissuse of acute paraquat-induced rats

目的检测百草枯(PQ)诱导急性肺损伤(ALI)中肺组织热休克蛋白70(HSP70)的表达情况。方法健康雄性SD大鼠36只,随机分为对照组(n=18),ALI组(n=18)。ALI组用PQ灌胃(200 mg/kg)染毒建立大鼠ALI模型。对照组用等量生理盐水灌胃。采用RT-PCR检测大鼠肺组织HSP70的表达,比色法检测肺组织髓过氧化物酶(MPO)、伊文蓝(EBD),观察肺组织病理变化。结果 ALI组大鼠肺组织HSP70的表达在染毒后12 h明显增高,差异有统计学意义(P<0.05),24 h达高峰。MPO及EBD水平显著增高(P<0.01)。肺组织病理检查可见PQ中毒后肺泡壁充血、炎性细胞浸润,并有局灶性出血。结论 HSP70在PQ中毒后的表达增多,可能参与了PQ诱导的ALI后的组织保护作用。

Objective To examine the expression of HSP70 in acute lung injury（ALI） induced by paraquat（PQ）.Methods Thirty-six healthy male SD rats were randomly divided into two groups：control grorp and ALI grorp.PQ intragastrically poisoning at the dose of 200 mg/kg made a model of the acute lung injury in Sprague-Dawley（SD） rats.The expression of HSP70 in lung tissue was assayed by RT-PCR.Myeloperoxidase（MPO） and Evens blue dye（EBD） in lung tissues were measured by colorimetry.Pathology of lung tissue was observed.The data were handled by the analysis of vanance and NK method using SPSS 12.0.Results Compared with control group,the expression level of HSP70 in rats of ALI group increased significantly at any time point,peaking at 24 h post poisoning（P0.05）.The levels of MPO and EBD in ALI group significantly increased compared to controls at 24 h after PQ posioning（P0.01）.The pathological changes in lungs of PQ poisoned rats mainly showed congestion,leukocytes infiltration and local hemorrhage.Conclusion HSP70 may play a role in the protection of ALI induces by PQ.