摘要
目韵探讨外源性纤溶酶原激活物抑制因子-1（PAI-1）对人肝星状细胞（HSC）转化生长因子β1（TGFβ1）、基质金属蛋白酶抑制因子-1（TIMP-1）及透明质酸（HA）表达的影响。方法培养液中加入PAI-1，采用四甲基偶氮唑盐法检测人HSC株LX-2的增殖变化，确定PAI-1的最佳干预浓度。将LX-2培养液中加入PAI-1分别墙养12、24、48h，采用酶联免疫吸附法（ELISA）检测细胞上清液中TGFβ1、TIMP-1及HA的变化。结果阴性对照组A492为0．473±0．035；PAI-1浓度为5、10、20、40、80μg／L时，A492分别为1．249±0．440、1．636±0．315、1．283±0．413、0．938±0．263、0．303±0．125，PAI-1浓度为10μg／L时刺激作用最为明显（F=11．697，P〈0．01）。加入PAI-1培养12、24、48h组较阴性对照组细胞上清液中TGFβ1、HA及TIMP-1表达增加（F=1566．752、P〈0．01，F=235．632、P〈0．01，F=67．359、P〈0．05）。结论PAI-1可促进肝星状细胞TGFβ1、TIMP-1及HA的表达，从而影响肝纤维化的发生发展。
Objective To study the effect of plasminogen activator inhibitor type-1 （ PAI-1 ） on the protein expression of transforming growth factor-beta 1 （TGFβ1 ）, hyaluronic acid （HA） and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） of hepatic stellate cells （ HSC ）. Methods Methyl thiazolyl tetrazolium （MTT） was performed to detect the effect of PAI-1 on the proliferation of LX-2, and to determine the perfect intervention concentration of PAI-1. After incubation with PAI-1 in LX-2 culture solution for 12 h,24 h,48 h,the protein expression of TGFβ1 ,TIMP-1 and HA was observed with enzyme linked immunosorbent assay （ELISA）. Results A492 in the negative control was 0. 473 ± 0. 353, and 1. 249 ±0. 440, 1. 636 ±0. 315,1. 283 ±0. 413, 0. 938 ± 0. 263 and 0. 303 ± 0. 125 when the concentration of PAI-1 was 5,10,20,40 and 80 μg/L, respectively, a concentration of 10 μg/L of PAI-1 showed the strongest stimulation remarkablely （F = 11. 697, P 〈 0. 01 ）. After incubation with PAI-1 for 12 h,24 h,48 h,the protein expression of HA,TGFβ1 and TIMP-1 in the LX-2 supernatant significantly increased compared to negative control （F = 1566. 752,235.632 and 67. 359, respectively,P 〈 0. 01 or 〈 0. 05 ）. Conclusion PAI-1 may influence the development of hepatic fibrosis through promoting the protein expression of HA ,TGFβ1 and TIMP-1 of HSC.
出处
《中国综合临床》
2011年第3期232-235,共4页
Clinical Medicine of China
基金
太原市民生科技计划项目（100173）
