蚕丝固定化脂肪酶催化猪油水解

Hydrolysis of Lard by Immobilized Lipase on Silk

研究了蚕丝固定化脂肪酶催化猪油水解的性能。结果表明：在温度为５５℃、ｐ Ｈ 为 ８２ 的条件下，固定化脂肪酶活性最高。底物浓度高达５５％ （ Ｗ ／ Ｖ）时，仍无底物抑制。适宜的搅拌速度是４５０ ｒ／ｍ ｉｎ～５００ ｒ／ｍ ｉｎ。在上述条件下，固定化脂肪酶间歇水解猪油时的操作半衰期约为 ２６０ ｈ。

The hydrolysis of lard by immobilized lipase on silk has been studied.The highest activity of immobilized lipase can be attained at temperature of 55℃ and pH value of 8.2.No inhibition was found as the substrate concentration increased up to 55%(W/V).The optimal rate of stirring is between 450 r/min～500rpm.The operational half life of immobilized lipase is about 260 hours when batch hydrolysis of lard is catalyzed by it on the optimal conditions.