四种染色法在幽门螺杆菌检测中的应用及比较

Application and comparison of four staining methods on helicobacter pylori detection

目的:探讨四种染色法在幽门螺杆菌检测中的应用及比较。方法:收集88例确诊为慢性浅表性胃炎及胃溃疡合并幽门螺杆菌感染患者,分别采用Warthin-starry染色法、碱性品红法、改良Giemsa法和硼酸美蓝法检测胃粘膜幽门螺杆菌,比较这四种检测方法对幽门螺杆菌检测的阳性率。结果:与其他三种染色法相比,Warthin-starry染色法对幽门螺杆菌检测阳性率较高,差别具有统计学意义(P<0.05)。结论:与其他三种染色法相比,Warthin-starry染色法对幽门螺杆菌检测阳性率较高,值得临床推广。

Objective： To investigate and compare four staining methods on helicobacter pylori （HP） detection. Method： We enrolled 88 patients diagnosing as chronic superficial gastritis and gastric ulcer with definitive HP infection, then we used W-S staining, aniline red （A-R） staining, modified Giemsa （M-G） staining and boracic acid methylene blue （BAMB） staining to evaluate HP infection of gastric mucosa and compared the positive detection rate of the above four methods. Results： Compared with the other three methods, the positive detection rate of liP with W-S staining was significantly higher （P〈0.05）. Conclusion： Compared with the other three methods, the positive detection rate of liP with W-S staining was higher and W-S staining deserved broadly application in clinic.