一株沃氏葡萄球菌耐药基因的研究

Study on the strain of staphylococcus warneri with multi-drug resistance gene

目的:研究沃氏葡萄球菌多重耐药情况和相关机制。方法:采用聚合酶链反应(PCR)法对一株多重耐药沃氏葡萄球菌进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、大环内酯-林可酰胺-链阳菌素B类(MLSB)基因、四环素类基因、糖肽类基因、消毒剂基因检测。结果:PCR结果显示该株的mecA,TEM,ermA,ermC,msrA,msrB,aac(6′)/aph(2″),qacA基因均为阳性,ant(4′,4″),aph(3′)-,TetM,vanA,vanB基因均为阴性。结论:该株沃氏葡萄球菌存在多重耐药基因,而且对胺类、胍类消毒剂耐受。

Objective：To study the instance and mechanism of multi-drug resistance of Staphylococcus warneri.Methods：The genes of multi-drug resistance including beta-lactamase,aminoglycosides modifying enzymes,macrolide-Lincomycin amide-streptogramin（MLSB）,tetracycline,glycopeptides,disinfect resistance were determined by polymerase chain reaction（PCR）.Results：The results of PCR indicated that mecA,TEM,ermA,ermC,msrA,msrB,aac（6′）/aph（2″）,qacA genes were positive,while ant（4′,4″）,aph（3′）-Ⅲ,TetM,vanA,vanB genes were negative.Conclusion：There are multi-drug resistance genes in the strain of Staphylococcus warneri,which resist disinfectant of amines and guanidines.