运动和限食对肥胖大鼠体脂及血清HDL-C和HL活性的影响

The Effects of Exercise and Food Restriction on Body Fat and Serum HDL-C and Hepatic Lipase Activity of the Obese Rats

目的:探讨运动和限食干预对食源性肥胖大鼠体脂及血清高密度脂蛋白胆固醇(HDL-C)和肝脂酶(HL)活性的影响。方法:肥胖模型建立后将32只肥胖大鼠随机分为对照组、限食组、运动组、运动+限食组。各组继续喂食高脂饲料,限食组喂食量为对照组的80%。游泳运动,5周,6 d/周,90 min/d。最后一次运动结束后60 h宰杀大鼠,测量体重、肾脏周围脂肪重量、附睾周围脂肪重量,血清HDL-C浓度和HL活性。结果:(1)对体重降低的影响:限食(P<0.01)、运动(P<0.001)有显著作用;运动与限食之间无协同作用(P>0.05)。(2)对内脏脂肪重量降低的影响:限食无显著影响(P>0.05);运动影响作用显著,大鼠肾脏周围脂肪(P<0.05)和附睾周围脂肪重量(P<0.01)降低;运动与限食之间无协同作用(P>0.05)。(3)对于血清HDL-C浓度升高的影响:限食(P<0.05)、运动(P<0.01)有显著作用;且运动与限食在大鼠血清HDL-C浓度升高的变化中产生了协同作用(P<0.01)。(4)对于血清HL活性降低的影响:限食作用不显著(P>0.05);运动影响作用显著(P<0.05);运动与限食之间无协同作用(P>0.05)。结论:在不改变膳食结构的前提下,运动和限食能够显著降低肥胖大鼠体重,对内脏脂肪的堆积产生抑制作用,提高肥胖大鼠血清HDL-C浓度同时降低HL活性,降低发生动脉硬化病变的风险。

Objective： To discuss the influence of exercise and food restriction on body fat and serum HDL-C and hepatic lipase activity of obese SD rats.Methods： After the models were prepared successfully,32 obese rats were randomly divided into four groups： normal control group（NC）,food restriction group（FR）,exercise group（E） and exercise＋food restriction group（E＋FR）.They were continued to be fed with high fat die.Food restriction groups were given 80% high fat diet referring to control group.Drinking freely.The exercise form is swimming for 5 weeks,6 d/w,90 min/d.Sixty hours after last exercise,all rats were put to death.Weight、circumrenal fat、epididymis fat、serum HDL-C and hepatic lipase activity were detected.Results：（1） Food restriction and exercise had a remarkable influence on the weight of obese SD rats（P0.01,P0.01,respectively）,but there were no interaction between them（P0.05）;（2） Food restriction had no remarkable influence on circumrenal fat and epididymis fat of obese SD rats（P0.05）;Exercise had a remarkable influence（P0.05,P0.01,respectively）;There were no interaction between them（P0.05）;（3） Food restriction and exercise had a remarkable influence on serum HDL-C of obese SD rats（P0.05,P0.01,respectively）;There was an interaction between Exercise and food restriction,which increased serum HDL-C significantly（P0.01）.（4） Food restriction had no remarkable influence on hepatic lipase activity（P0.05）;Exercise had a remarkable influence（P0.05）;there were no interaction between exercise and food restriction（P0.05）.Conclusion： Without changing diet structure,exercise and food restriction could reduce the weight of the obese SD rats significantly,and could inhibit the accumulation of visceral fat,increase serum HDL-C,lower hepatic lipase activity.The risk of arteriosclerosis might be decreased.