摘要
群众杨插条苗经 PEG渗透胁迫处理后 ,取根部样品 ,经过快速冷冻、冷冻干燥、乙醚真空渗透及塑料包埋后 ,用透射电镜 X-射线能谱微区分析技术 ,对根表皮层、皮层及中柱薄壁组织细胞的细胞壁、细胞质和液泡中的 K+含量与分布进行了测定。结果表明 ,在渗透胁迫下 ,在根各部位组织细胞的细胞壁、细胞质和液泡中 ,K+含量都有不同程度的增加 ,增加的 K+主要积累在液泡中。渗透胁迫所促进的 K+吸收可被蛋白质合成抑制剂环己酰亚胺所抑制。
Cuttings of Populus × xiaozhuanica W.Y.Hsu et Liang cv.‘ Popularis ’ 35 44 were treated with PEG solution. After fast freezing,freeze drying,pressure infiltration with ether and embedding in plastic,K + content were measured by X ray microanalysis in cell wall,cytoplasm and vacuole of rhizodermis, cortex and stelar parenchyma cells of roots. Compared to the controls,K + content in all root tissues were increased by osmotic stress.In particular,the increasing K + was largely accumulated in vacuole. The K + accumulation induced by osmotic stress could be inhibited completely by cycloheximide.
出处
《林业科学研究》
CSCD
北大核心
1999年第4期346-349,共4页
Forest Research
基金
1996~ 1998年国家自然科学基金资助课题"干旱胁迫下杨树气孔运动机制研究"的部分内容
关键词
群众杨
根细胞
K^+累积
渗透胁迫
微区分析
X线
poplar
K^+ accumulation in root
osmotic stress
cycloheximide
X-ray microanalysis