木麻黄弗兰克氏菌的菌剂类型与保藏方法对接种效果的影响

Effect on Inculation of Forms and Storage Methods of Casuarina Frankia inoculum

用弗兰克氏菌 ( Frankia) 90 2 2菌株和 2 87菌株的 3种菌剂类型、2种保藏方法对木麻黄苗木接种效果影响的研究结果表明 :90 2 2菌株的 3种菌剂类型以室温保藏 6～ 9个月的蛭石菌剂的接种效果最好 ,2 87菌株则以 4℃保藏 6～ 9个月的干海藻酸钙菌剂的接种效果最好 ,其苗高生长、生物量、结瘤量和侵染率最高。室温保藏的 90 2 2菌株和 2 87菌株的干海藻酸钙菌剂接种效果最差。应用MPN方法测定了经过 2 4个月室温保藏的 90 2 2菌株蛭石菌剂的活菌数量为 4.8× 10 3个 .g-1。

Significant advances have been made in the development of peat based,vermiculite based and alginate bead Frankia inoculum. Investigations of storage conditions for peat,vermiculite and alginate Frankia inoculum showed that the inoculant of Frankia 9022 in vermiculite maintained high viability and infectivity for up to 6 months when stored at room temperature;the inoculant of Frankia 287 in alginate bead maintained high viability and infectivity for up to 6 months when stored at 4℃. Determination the number of potentially infective units for Frankia 9022 vermiculite inoculant of 24 months storage at room temperature were 4.8×10 3/g inoculant.