+Gz 重复暴露对大鼠脑组织热休克蛋白70 基因表达的影响(英文)

Expression of HSP70 Gene in Rat Brain after Exposures to Repeated +Gz

目的探讨＋ Ｇｚ 重复暴露后大鼠脑组织热休克蛋白７０（ Ｈ Ｓ Ｐ７０） 基因表达的变化及其在＋ Ｇｚ所致脑损伤中的作用。方法用半定量反转录聚合酶链反应（ Ｒ Ｔ－ Ｐ Ｃ Ｒ） 检测方法检测＋ Ｇｚ 重复暴露大鼠脑组织 Ｈ Ｓ Ｐ７０ ｍ Ｒ Ｎ Ａ 表达水平。结果 ＋ Ｇｚ 重复暴露后３０ ｍｉｎ 、６ ｈ 大鼠脑组织 Ｈ Ｓ Ｐ７０ｍ Ｒ Ｎ Ａ 均有升高，分别是对照组的１ ．７ 倍和２ ．６ 倍，２４ ｈ 恢复正常。结论 ＋ Ｇｚ 重复暴露可诱导大鼠脑组织 Ｈ Ｓ Ｐ７０ ｍ Ｒ Ｎ Ａ 的表达， 可能与＋ Ｇｚ 所致脑损害后神经细胞的自身保护有关。

Objective To study the changes of mRNA expression of heat shock protein 70 (HSP70) in the rat brain exposed to repeated +Gz. Method The mRNA expression levels of HSP70 in rat brain were measured by semi quantitative reverse transcription polymerase chain reaction (RT-PCR). Result The HSP70 mRNA expression levels in rat brains taken 30 min and 6 h after repeated +Gz exposures were significantly higher than those in control group, while the difference between the levels of control group and those of experimental rat brains taken 24 h after +Gz exposure was not significant. Conclusion It is suggested that HSP70 mRNA expression in rat brain can be induced by repeated +Gz exposures and the increased HSP70 mRNA expression may play an important role in self protection against brain damage induced by+Gz exposures.