肉苁蓉不同成分抗衰老作用的研究

Desertliving cistanche anti-aging action:A comparative study

目的:研究肉苁蓉不同成分对致衰老大鼠增龄性变化的影响。方法:用D-半乳糖制备衰老模型大鼠,连续灌服肉苁蓉不同成分6周,测定肝脏Ca2+-ATP酶活性、肝线粒体MDA含量、膜流动性、PLA活性。结果:肉苁蓉各成分显著提高衰老模型大鼠肝脏Ca2+-ATP酶活性,肝线粒体膜流动性,显著降低肝线粒体MDA含量、PLA2活性,肉苁蓉水提液的效果最明显。结论:肉苁蓉不同成分可以通过提高肝脏抗氧化能力,减少肝线粒体氧化损伤,起到延缓衰老的作用,且肉苁蓉水提液的作用优于醇溶成分和多糖。

Objective：To investigate the age-related changes in anti-oxidation of rat liver mitochondria induced by D-gal and the anti-aging effects of desertliving cistanche and its water extract,ethanol solution and polysaccharide components at the level of mitochondria.Methods：We used the aging rat induced by D-gal as research sample.We measured the Ca2＋-ATPase activity in rat liver and determined the MDA content,membrane fluidity and PLA2 activity in rat liver mitochondria in an attempt to study the effects of different components of desertliving cistanche on the above-mentioned indexes.Results：The MDA content and PLA2 activity were markedly decreased,and the Ca2＋-ATPase activity and membrane fluidity were obviously increased by giving the water extract,ethanol solution and polysaccharide components to the desertliving cistanche groups（P〈0.05,P〈0.01）.In the water extract group,the water extract was able to cause the above indexes up to the level of the young group but the effects of ethanol solution and polysaccharide components were relatively inferior.Conclusion：Desertliving cistanche different components can postpone aging by raising the liver anti-oxidation function,reducing the damage by the peroxidation of the liver mitochondria,with the effect of desertliving cistanche water extract better than that of other components.