摘要
目的:研究齐墩果酸对宫颈癌Hela细胞增殖的影响;初步探讨齐墩果酸诱导Hela细胞凋亡的作用机制。为临床治疗宫颈癌提供实验和理论依据。方法:MTT法检测OA对宫颈癌Hela细胞增殖的影响。HE染色检测OA对宫颈癌Hela细胞凋亡的影响。结果:OA以时间和剂量依赖性的特点抑制宫颈癌Hela细胞的增殖。不同程度地显示典型的细胞变圆,细胞核深染,染色质边集、呈环状、团块或新月体状,以及细胞浆浓缩和"发芽"现象等细胞凋亡的形态学特征。结论:OA以时间和剂量依赖性抑制宫颈癌Hela细胞的增殖,其作用机制与改变细胞周期分布、促进细胞凋亡有关。
Objective：To study the inhibitory effect of oleanolic acid on Hela cells and investigate the mechanism that induced Hela cell apoptosis in an attempt to provide laboratory data for its application in the prevention and treatment of cervical cancer.Methods：Hela cells were treated with various concentrations of oleanolic acid for 24h,48h and 72h,respectively,and the cell proliferation was detected by using the MTT assay.Results：Oleanolic acid inhibited the proliferation of Hela cells in a dose and time-dependent manner.Conclusion：Oleanolic acid can inhibit the growth of Hela cells,and the mechanism may be related to its blockage of cell cycle and apoptosis induction.
出处
《黑龙江医药科学》
2011年第1期31-32,共2页
Heilongjiang Medicine and Pharmacy
关键词
齐墩果酸
宫颈癌
细胞凋亡
cervical cancer
oleanolic acid
apoptosis
