摘要
研究了不同的因素对纤维素酶酶解玉米秸秆的影响,包括时间、反应温度、底物浓度、酶用量等。采用5因素和4水平进行玉米秸秆酶解正交试验。结果表明,影响玉米秸秆酶解的因素为反应温度>水解时间>底物浓度>速度>酶用量;得到纤维素酶酶解玉米秸秆的最佳条件为反应时间48h,酶解温度55℃,底物浓度20g/L,速度130r/min,酶用量为200U/g。该研究为玉米秸秆的生物利用提供了理论依据。
Effects of different factors, such as treating time, temperature, concentrations of substrate and enzyme, on efficiency of enzymatic hydrolysis of corn stalk by cellulose were studied. The orthogonal test with 5 factors and 4 levels was applied. The results showed that significance of factors ordered as follows： reaction temperature 〉hydrolysis time 〉concentration of substrate 〉 shaking speed 〉concentration of enzyme. The optimum conditions for degradation of corn stalk with cellulase were： reaction temperature of 55℃, hydrolysis time of 48h, concentration of substrate of 2%, shaking speed of 130r/min, enzyme concentration 200U/g. The research provided reference for biological utilization of corn stalk.
出处
《中国酿造》
CAS
北大核心
2011年第3期24-27,共4页
China Brewing
基金
973计划前期项目(2009CB226108)
湖南省教育厅优秀青年项目(10B045)
作物种质创新与资源利用国家重点实验室培育基地重点项目(10KFXM02)
关键词
纤维素酶
酶解
玉米秸秆
正交试验
cellulase
enzymatic degradation
corn stalk
orthogonal test
