期刊文献+

单糖和羧酸盐对酸性蛋白酶热稳定性的影响

Effects of monosaccharides and carboxylates on thermal stability of acid protease
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摘要 文中应用动力学方法,较系统地研究了酸性蛋白酶的热稳定性。试验结果显示某些单糖及羧酸盐能够明显延长该酶在较高温度下的半寿期,其中甘露糖和半乳糖均可延长酶的半寿期达11倍以上,能够起到有效保护酸性蛋白酶的作用。 The thermal stability of acid protease was studied with dynamic methods. The results showed that some monosaccharides and carboxylates could greatly increase the half-life of acid protease at a higher temperature. Mannose and galactose could extend the enzyme's half-life over 11 times, so they could play a visible role in the effective protection of acid protease.
作者 吴京平
出处 《中国酿造》 CAS 北大核心 2011年第3期125-128,共4页 China Brewing
关键词 酸性蛋白酶 单糖 羧酸盐 半寿期 热稳定性 acid protease monosaccharide carboxylate half-life thermal stability
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参考文献5

  • 1COLACO C, SEN S, THANGAVELU M, et al. Extrordinary stability of enzymes dried in trehalose: simplified molecular biology[J]. Nat Bioteeh- nol, 1992(10): 1007-1011.
  • 2PAUL SD, FINN W, ROBERT BW, et al. Stabilization to Heat and protease degradation by complexing with antibody and by chemical modification[J]. Biochim Biophys Aeta, 1974, 350(2): 432.
  • 3KELLIS JT, NYBERG K. Contribution of hydrophobic interactions to protein stability[J]. Nature, 1988, 333: 784-786.
  • 4FONTANA A. Structure and stability of thermophilic enzymes.Studies on thermolysin[J]. Biophys Chem, 1998, 29(1-2): 181-193.
  • 5MALIKKIDES CO, WEILAND RH. On the thermal denaturation of glucose oxidase[J]. Bioteehnol Bioeng, 1982, 24(8): 1911-1914.

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