摘要
丙酮酸脱羧酶是酿酒酵母代谢中非常关键的酶,其催化的反应是利用丙酮酸生成乙醛。研究构建了一种分光光度法测定酿酒酵母中丙酮酸脱羧酶活性的方法,在25℃和pH6.0的条件下测定反应液5min内在波长340nm处每分钟吸光度值的变化。以在25℃和pH6.0的条件下每分钟转化1.0μmol丙酮酸生成乙醛来定义酶的活性。
Pyruvate decarboxylase, which catalyzes pyruvate to acetaldehyde, is a key enzyme in metabolic of Saccharomyces cerevisiae. A method for determination of pyruvate decarboxylase activity yeast with spectrophotometry was established in this paper. Changes of absorbance under 340nm per min at 25℃, pH 6.0 were measured. One unit of activity of enzyme was defined as conversion 1.0μm of pyruvate to acetaldehyde per minute under the conditions ofpH 6.0, 25℃.
出处
《中国酿造》
CAS
北大核心
2011年第3期128-130,共3页
China Brewing
关键词
酿酒酵母
丙酮酸脱羧酶
测定
Saccharomyces cercvisiae
pyruvate decarboxylase
determination
