DCC缩合酯化法合成水杨酸低聚壳聚糖酯及抑菌性研究

Study on the Preparation of Salicylate Ester of Chitooligosaccharide by DCC Condensation Method and Its Anti-bacterial Function

在保留C2-NH2抗菌活性基团的基础上,以DCC为脱水剂制备了具有双抑菌活性中心的水杨酸低聚壳聚糖酯(SEC),并对其抑菌活性进行了研究。结果显示,SEC最佳合成反应条件为:n(低聚壳聚糖)∶n(水杨酸)∶n(DCC)=1∶4∶4,冰浴条件下酯化反应48 h,产物酯化度接近73%。该条件下制备的壳聚糖衍生物对各种常见菌的抑制效果明显增强,而且抑菌范围变宽。

Retaining the amine group and taking N,N＇-dicyclohexylcarbodiimide（DCC） as a dehydration agent,a salicylic ester of chitooligosaccharide（SEC） with two antibacterial active sites was synthesized and its antibacterial and antifungal activities were studied.The experimental results showed that the optimum reaction conditions were： n（chitooligosaccharide）∶n（salicylic acid）∶n（DCC）=1∶4∶4 and reacting for 48 h in ice bath.The esterification degree of SEC prepared under this condition could reach 72%,and it has high efficiency and broad anti-bacterial activity.