人参皂苷Rb1对胎鼠背根神经节损伤的保护作用

The effect of ginsenoside Rb1 on injured dorsal root ganglion neurons

目的观察不同浓度人参皂苷Rb1对损伤胎鼠背根神经节(DRG)神经元形态学改变的影响,探讨人参皂苷Rb1对谷氨酸引起的兴奋性神经毒损伤的保护作用,为Rb1的进一步研究和临床应用提供理论依据。方法选择胎龄为15 d的SD大鼠,获取DRG神经元并进行体外分散培养48 h后,随机分为对照组、谷氨酸损伤组、谷氨酸损伤+低浓度Rb1(10μg/ml)保护组和谷氨酸损伤+高浓度Rb1(100μg/ml)保护组,继续培养12 h。终止培养后,倒置相差显微镜观察各组神经元的生长状态,MTT检测不同浓度人参皂苷Rb1孵育的背根神经节神经元的凋亡增殖情况。结果对照组DRG神经元细胞贴壁呈单层散在分布,少部分出现细胞聚集现象,突起较长且互相交织形成网状;谷氨酸损伤组DRG神经元细胞聚集现象明显,神经元突起变短、断裂甚至消失;谷氨酸损伤+Rb1保护组DRG神经元细胞部分呈簇状聚集,部分呈单个散在分布,突起仍然相互交织。MTT结果显示谷氨酸损伤+Rb1保护组细胞存活率均高于谷氨酸损伤组,但高浓度Rb1保护组与低浓度Rb1保护组之间差异无统计学意义。结论人参皂苷Rb1可以影响损伤胎鼠DRG神经元的形态学改变和凋亡增殖情况,对胎鼠背根神经节神经元具有一定的保护作用。

Objective To observe the effect of ginsenoside Rb1 on the morphology of injured dorsal root ganglion（DRG） neurons.Methods The DRG neurons of SD rat embryos were cultured for 48 h,then all cultured cells were divided into 4 groups randomly.In the control group,nothing was added to the cultures.In the injured group,glutamic acid was added.In the Rb1 group,additional Rb1 were added.After culture,the morphological changes of neurons in each group were observed and the percentage of living neurons was determined.Results In the control group,the DRG neurons scattered in a single layer,a little part gathered together,the neurite was longer than other group and cross-cutting a mesh.In the injured group,most cells were gathered together,the processes became shorter or even disappeared,some neurite was broken.In the Rb1 group,some neurons gathered together and others singled,neurite networks still exist.MTT result showed that the cell survival rate in the Rb1 group was higher than that of the injured group,but there is no difference between the high concentration group and the low concentration group.Conclusion Ginsenoside Rb1 can affect the morphological changes of injured DRG neurons,it has neuroprotective effect on the neurons.