老年患者2型糖尿病并脑梗死与eNOS基因多态性的关系研究

Association between polymorphism of endothelial nitric oxide synthase gene in type 2 diabetes with cerebral infarction in the aged

目的探讨内皮细胞性一氧化氮合酶(eNOS)基因内含子4上a/b基因的多态性与老年患者单纯2型糖尿病及2型糖尿病并脑梗死(cerebral infarction,CI)的关系。方法应用聚合酶链反应(PCR)技术,检测109例老年患2型糖尿病合并CI病人的基因型,并与对照组比较。结果 2型糖尿病合并CI组eNOS基因ab基因型的频率(26.61%)明显高于对照组(12.71%)(P<0.01)及2型糖尿病组(12.94%)(P<0.05),a等位基因的频率(13.30%)也明显高于对照组(6.78%)及2型糖尿病组(6.47%)(P<0.05),均有统计学意义。结论 eNOS基因ab基因型与老年人2型糖尿病合并CI有相关性,等位基因a可能是2型糖尿病合并CI的危险因素。

Objective To investigate association between the a/b in intron 4 polymorphism of endothelial nitric oxide synrhase（eNOS） gene and type 2 diabetes with cerebral infarction（CI） in the aged.Methods The genotypes of 109 aged patients type 2 diabetes with CI and control group and type 2 diabetes group was performed by polymerase chain reaction（PCR）.Results The ab genotype distribution frequency of eNOS gene in diabetes with CI group was higher than control group（26.61%vs12.71%）（P〈0.01）.higher than that of type 2 diabetes（12.94%）（P〈0.05）too.The a allele frequency of eNOS gene of diabetes with CI group is significantly higher than control group（13.30%vs6.78%）,is significantly higher than type 2 diabetes（6.47%）too.Statistical differences between diabetes with CI group and control group were significant（P〈0.05）,and between diabetes with CI group and diabetes group（P〈0.05）were significant too.Conclusion There is association between ab genotype of eNOS and diabetes with CI in the aged.The eNOS a gene may be a risk factor for diabetes with CI.