细胞融合法获取耐高温絮凝酵母研究(Ⅰ)高温大曲酒曲中耐高温酵母的分离

Studies on high temperature endurable flocculating yeast obtained by protoplast fusion technique(I) Isolation of high temperature endurable yeast from thermal daqu

为提高菌种的耐温性 ,获得有耐高温性状的酵母 ,采用细胞融合法构建耐高温絮凝酵母 ,从高温大曲酒曲中用麦芽汁富积、分离酵母 ,并对分离出的几株菌种进行了酒精发酵监测 ,耐酸、耐酒能力测定 ,以及耐受温度和最适生长温度的测定 ,最终得到一株耐高温的酿酒酵母 (Saccha romycesCerevisiae) ,其耐受温度达 70℃ ,耐酸 pH 2 5～ 8 0 ,耐酒体积分数为 12 % ,最适生长温度40～ 42℃ ,发酵实验含酒量体积分数为 5 7%。它可做为细胞融合的一个亲本 ,以便获得耐高温性状。

This are going to apply the method of protoplast fusion to construct high temperature endurable flocculating yeast.In the experiment high temperature endurable yeasts are accumulated by malt extract medium and then separated from temperature Daqu.The isolated strains are experimented for alcohol fermentation and last a strain is obtained.Its dead temperature is 70℃,pH 2.5-8.0,endurable alcohol 12%,optimum temperature 40-42℃and fermentation alcohol rate is 5.7%.