丁基苯酞对血管性痴呆小鼠行为学及海马神经元ICAM-1表达的影响

The Effect of NBP on the Behaviors and the Expression of ICAM-1 in Hippocampus of Mice with Vascular Dementia

目的观察丁基苯酞(NBP)注射液对血管性痴呆(VD)小鼠行为学及海马神经元细胞间黏附因子-1(ICAM-1)蛋白表达的影响。方法采用间断阻断小鼠双侧颈总动脉血流,同时给予尾部放血的方法,制备VD模型,同时设立假手术组及丁基苯酞治疗组,应用跳台实验、水迷宫实验检测小鼠的学习和记忆成绩,应用免疫组织化学染色法检测小鼠海马CA1区神经元ICAM-1表达水平。结果 VD小鼠学习、记忆能力较假手术组明显降低(P<0.05),出现认知功能障碍,海马组织中ICAM-1表达明显增加(P<0.01),给予丁基苯酞治疗后,学习、记忆能力较模型组提高,认知功能明显改善(P<0.05),且海马CA1区ICAM-1表达明显降低(P<0.01)。结论丁基苯酞可通过减少ICAM-1在海马神经元中的表达,抑制VD的炎症反应,从而减轻VD小鼠的脑损伤程度,改善VD小鼠的认知功能障碍。

Objective To observe the effects of 3-n-butylphthalid（NBP） on the behaviors and the expression of ICAM-1 in hippocampus of vascular dementia（VD） model.Methods Kunming mice were divided randomly into three groups：model group,sham-operated group and treated group（NBP,7.5 mg·kg-1·d-1）.The mice model was established by repeated occlusion and reperfusion of the bilateral common carotid arteries and ischemic hypotension for three times.The capabilities of learning and memory of mice were investigated by the step down test and water maze test,the change of ICAM-1 exprssion in hippocampus CA1 in each group was detected by immunohistochemistry technique.Results Compared with sham-operated group,the abilities to learning and memory in model group decreased markedly（P0.05）,and the number of immunoreactive positive neurons of ICAM-1 increased significantly（P0.01）.The grades of learning and memory in NBP-treated group ameliorated apparently compared with model group（P0.05）,and the number of immunoreactive positive neurons of ICAM-1 decreased meanwhile（P0.01）.Conclusion NBP could inhibit the inflammatory response in VD by reducing the expression of ICAM-1 in hippocampus,thereby reduced the extent of brain damage and improved the clinical symptoms of VD.