卡介菌多糖核酸对PMA诱导的U937细胞功能的影响

Effects of BCG-PSN on U937 cells stimulated by PMA

目的研究卡介菌多糖核酸(BCG-PSN)对PMA刺激的U937细胞分泌炎性因子TNF-αI、L-1β的影响。方法人髓系白血病细胞U937用PMA诱导成熟后分5组:正常对照组、LPS组、高剂量、中剂量、低剂量BCG-PSN组,培养24 h后收集上清,ELISA测定细胞培养上清中TNF-α、IL-1β的浓度。结果 BCG-PSN刺激PMA诱导的U937产生TNF-α明显增多,与正常对照组相比,差异有统计学意义(P<0.05)。BCG-PSN刺激PMA诱导的U937产生的IL-1β与正常对照组相比,差异无统计学意义。结论 BCG-PSN可以刺激PMA诱导的U937细胞产生TNF-α,对U937产生的IL-1无明显的影响。

Objective To study the effect of BCG-PSN on the inflammatory cytokine TNF-α and IL-1β secreted from macrophage U937.Methods Human mononuclear macrophage U937 were cultured and induced to be mature by PMA in vitro,and then divided into five groups： control group,LPS group,low dose BCG-PSN group,middle dose BCG-PSN group,high dose BCG-PSN group.TNF-α as well as IL-1β released from the U937 cells were detected by ELISA.Results The level of TNF-α released from the stimulated U937 cells in BCG-PSN groups increased significantly than that in the control group（P0.05）;while the level of IL-1β in BCG-PSN groups had not significant difference with that in the control group.Conclusions U937 cells could be activated to increase the secretion of TNF-α by BCG-PSN.BCG-PSN has not effect on the secretion of IL-1β.