紫外激光等选育生淀粉糖化酶高产菌株及其应用

SCREENING OF HIGH RAW STARCH GLUCOAMYLASE PRODUCING STRAIN BY UV AND He Ne LASER IRRADIATION AND ITS APPLICATION

以紫外线、ＬｉＣｌ、５氟尿嘧啶、ＨＮＯ２ 为诱变剂，黑曲霉Ｓｘ 为出发菌株，选育出生淀粉糖化酶酶活力提高１２％ 的突变株黑曲霉Ｓ′ｘ制取黑曲霉Ｓ′ｘ 的原生质体，用ＨｅＮｅ 激光照射，经反复筛选得到突变株黑曲霉Ｓｙ，其酶活力较出发菌株提高５１％ ，而酸性蛋白酶活力降低４５％

A mutant strain A niger Sx′ with higher yield raw starch glucoamylase was obtained from wild strain of A niger Sx by UV,LiCl,5 Fu and HNO 2 treatment Its enzyme activity increased by 12% After many times of He Ne Laser irradiation to protoplast of A niger Sx′,A niger Sy was obtained Its raw starch glucoamylase activity increased 51% than that of the original strain A niger Sx,whereas A niger Sy showed reductions in acid protease productivity of 45% Furthermore,applications of A niger Sy on the treatment of the grain stalks studied